Sunday, 20 January 2019

Cannabis oil treatment (THC)

Dear Stephen, dear all!

What do you think about using cannabis oil with a high content of THC component to treat glioblastoma. (Exact component concentration is not known (but declared as high).

Based on recommendations of one doctor, the oil should be applied according to the following scheme (it's also known as Rick Simpson's scheme):
* first day - one drop x 2 times a day;
* then every three days increase intake by one drop in the morning and evening
* reach 24 drops (approximately 1 ml) and take this amount x 2 times a day.

There are a lot of wonderful stories about its anti-cancer effect in the Internet, especially in case of brain tumor. But then I found this research http://cancerres.aacrjournals.org/content/64/6/1943.long#sec-10.

"Treatment of the glioblastoma cell line U373-MG and the lung carcinoma cell line NCI-H292 with nanomolar concentrations of THC led to accelerated cell proliferation that was completely dependent on metalloprotease and epidermal growth factor receptor (EGFR) activity."

"In the light of these results, the use of cannabinoids in cancer therapy has to be reconsidered, because relatively high concentrations of THC induce apoptosis in cancer cells, whereas nanomolar concentrations enhance tumor cell proliferation and may, therefore, accelerate cancer progression in patients."

"Smoking of THC is the most effective route of delivery, as THC is rapidly absorbed after inhalation, and the effects become fully apparent within minutes. Pharmacological activity of smoked THC depends on the depth and length of inhalation. Maximum serum concentrations up to 267 ng/ml (850 nm) are measured after smoking THC, whereas maximum serum concentrations of oral or rectal administered THC or its derivatives as a drug are lower (35–350 nm). Here we observed a proliferative response of glioblastoma and lung cancer cells at concentrations of 100–300 nm THC, whereas THC at micromolar concentrations induced cell death in agreement with previous observations with neuronal cell types and immune cells. These findings indicate that the biological responses to cannabinoids critically depend on drug concentration and cellular context. Taken together, these results have to be taken into account when considering therapeutic applications of cannabinoids. The risk in the medical use of THC or cannabis for the treatment of patients with established tumors is the further acceleration of tumor growth due to the proliferative potential of cannabinoids."

Questions:

1. Has anyone tried to measure THC concentrationt in the blood? Are there any tests for home use that can show the quantitative content of this component in the blood? I know that there are tests for saliva and urine, but how to compare blood concentration and saliva/urine?

2. Perhaps it is easier to measure the THC concentration in one oil drop? And then to estimate what concentration it causes in the blood?

3. Do I understand correctly that THC has a cumulative effect when using daily and in principle it is real to achieve micromolar concentration after some time?

4. Is it possible to avoid the psychotropic effect with micromolar concentration?

5. Is THC effective by itself or only with radiation therapy or chemotherapy?

6. May be it's really better smoking if it allows to achieve more THC concentration? 

I am looking forward to your answers! Thank you!


And please accept my late congratulations with New Year! Let it be full of wonders! Health to all of you and your loved ones!

Irina

Recurrant anaplastic astrocytoma treatment options

Hey all,
Need suggestions for treatment options and supplementation for my brother
His clinical history is-
clinical history date remarks
diagnosed in                                                                                                         september 2009                               assumed to be begnin tumour
                                                      observation through mri 
Tumour surgical resection 18th feb 2016 tumour started to enhance in size
gross resection of more than 95% 
tumour size 7.2x5.5x5.1( anaplastic oligoastrocytoma)
RT PLUS  CONCURRENT TEMOZOLOMIDE 2ndAPRIL 2016 TO 17th MAY 2016 59.4 gy ,33 fractions plus 120 mg temozolomide
Temozolomide june 2016 -december 2016 6 cycles
Recurrence                                                  may-18         
reradiation +concurrant temozolomide 18th jul 18-16th aug 18 36gy, 20 fractions plus 120 mg temozolomide
temozolomide chemo 1st jan 19-5thjan 19 200mg per day for 5 days                              
HIS BIOMARKERS ARE-

BIOMARKER AND GENOMIC FINDING STATUS
MICROSATELLITE STATUS MS-STABLE
TUMOUR MUTATIONAL BURDEN  TMB-LOW (4 MUTS/MB)
ATRX  LOSS
IDH1 R132H
NOTCH1 A465T SUBCLONAL,E450K-SUBCLONAL,R353C-SUBCLONAL
SMARCA4 T910M
TP53 G245S,R273C
MGMT UNMETHYLATED(METHYLATION SCORE-0.49)
ANTIBODY TYPE RESULT
GFAP POSITIVE
IDH1 R132 POSITIVE
KI-67 POSITIVE PROLIFERATIVE INDEX APPROX 10-15%
FISH TEST
1P/19Q CO-DELETION NEGITIVE
EGFR NEGITIVE FOR EGFR AMPLIFICATION
PTEN LOSS POSITIVE (39.3% OF NUCLEI EXAMINED)
His current medications are-

Part of Day Medicine Name Medicine count Notes
PRE MORNING BROMELAIN 500 MG NOW 3 EMPTY STOMACH
TAB PAN 40 MG RT OD 1 STOP ON 30TH JAN 2019
MORNING BREAKFAST CURCUMIN TABLETS 3 NOT WITH AVASTIN OR TAGRISSO/TARCEVA
GLYCEROL 30 ML 1
DIAMOX 250 MG 1
METAFORMIN 500MG 1 TWICE DAILY FROM 1ST FEB
IBUPROFEN 200 MG 1
CBD OIL 2 FULL DROPPERS 1
LEVIPILL 750 MG RT 1
LASILACTONE 20/50 MG RT 1
TAB DEXA 4 MG RT 1 STOP AFTER 21ST JAN
TAB BACLOFEN 5MG RT 1
REFRESH EYE DROP 2 BOTH EYES 2 DROPS
LEVOLIN NEBULISATION .63MG 1 STOP ON 25TH JAN 2019
TAB GLYCOPYRROLATE 1 MG 1 STOP ON 20TH JAN
TO BE ADDED VALGANCICLOVIR 450 MG 2
CHLOROQUINE 250 MG 1
CELEBRAX 200MG 1
WHEY PROTIEN 2 SCOOPS  WHEN RECD FROM USA
GRAPESEED EXTRACT 250 MG 1
STRESS B COMPLEX 2
LUNCH CURCUMIN TABLETS 3
MEBENDAZOLE 100 MG 1 START DOXYCYCLINE ON 18 TH APRIL
GLYCEROL 30 ML 1
IBUPROFEN 200 MG 1
LASIX 40 MG 1
REFRESH EYE DROP 2 DROPS STOP AFTER 21ST JAN
LEVOLIN NEBULISATION .63MG 1 TIME STOP ON 25TH JAN 2019
TAB GLYCOPYRROLATE 1 MG 1 STOP ON 2O TH JAN
TO BE ADDED CELEBRAX 200MG 1
NIGHT CURCUMIN TABLETS 3
ATORVASTATIN 40 MG 1 80 MG FROM 1ST FEB
GLYCEROL 30 ML 1
DIAMOX 250 MG 1
IBUPROFEN 200 MG 1
CBD OIL 2 FULL DROPPERS 1 AFTER HALF HOUR GIVE THC 6 DROPS
THC OIL 6 DROPS 1
LEVIPILL 750 MG 1
LASILACTONE 20/50 MG 1
TAB BACLOFEN 5MG RT 1
REFRESH EYE DROP 1 BOTH EYES
LEVOLIN NEBULISATION .63MG 1 TIME STOP ON 25TH JAN 2019
TAB GLYCOPYRROLATE 1 MG 1 STOP ON 2O TH JAN
TO BE ADDED VALGANCICLOVIR 450 MG 2 STOP ON 9TH FEB 2019
CELEBRAX 200 MG 1
BERBERINE 500 MG 1
BEDTIME ARTEMISIA 500 MG 2 WHEN RECD FROM PATRICE
TAB FRISIUM 5MG RT 1
PLEASE SUGGEST
1.SUPPLEMENTS TO ADD OR TO BE DISCONTINUED
2.AVASTIN OR LOUMUSTINE OR ANY OTHER TRIAL DRUG BEST SUITED FOR HIM
3.ANY OTHER EFFECTIVE THERAPY TO HELP HIS QUALITY OF LIFE.

Saturday, 19 January 2019

Phosphatidylserine

Stephen, are you familiar with the young man from Canada who is a long term survivor of GBM?

Apparently he took Phosphatidylserine but he is not intimating that is the reason for his survival.   Interesting website.



Do you have any thoughts on Phosphatidylserine?



Hope all is well.

Candy

Thursday, 17 January 2019

Temozolomide + mifepristone in rat glioma model


  • Mifepristone, an FDA and Health Canada approved antiprogestogen, used to induce abortion.
  • C6 rat glioma cells implanted into brains of male Wistar rats
  • temozolomide applied to the rats at a dose of 5 mg/kg/day intraperitoneally
  • mifepristone applied to the rats at a dose of 10 mg/kd/day subcutaneous injection
  • rat survival as show below ("sham" refers to rats undergoing sham surgery with no glioma cells implanted)
  • combination of temozolomide + mifepristone was effective where either single agent alone was  ineffective







Wednesday, 9 January 2019

advices on cocktails and corticoids

My father in France - 71 years old - has been diagnosed with a GBM grade IV in September 2018. Before that he has always been in good shape and was very sportive.
He has 3 tumors from 10mm to 40mm and seems to be inoperable. He already had 3 sets of TMZ and he is taking already some supplements I recommended based on my researches on Internet. 
Here is his complete current treatment started 1 month after first diagnosis (time to perform biopsy) :


Temodal3 cycles of 5 days done
Corticoids - Solupred50 to 60 mg / day
Cannabis suppositories1g / day (80% THC)
Chinese herbs and mushrooms (including Artemisia Annua)every day
Vitamin C5g / day
Nutraexpert Lipo HCA dietary suplements containing:
HCA1500 mg / day
Alpha Lipoic Acid1600 mg / day
Capsicum112 mg/ day
Quercetin45 mg/ day
Ginger46 mg/ day
Whey47 mg/ day
Green tea30 mg/ day
Nicotinamid20 mg/ day
Vitamin D325 µg/ 1000 UI
Ketogenic diet
Curcuma (Longvida) : added last week1g / day

Biopsy revealed :

  • IDH1 : negative
  • FGFR3 : negative
  • P53 protein : 15 - 20%
  • no information on MGMT

MRI performed early december has show a slight size increase in the 2 biggest tumors, the oncologist says it is not alarming as the treament started one month after first diagnosis.
Currently my father resists well and tries to keep daily walking activity. Next MRI will be next month. 

I just recently discovered the cocktail approach and your blog and I have some questions.

1. It seems many patients are taking Melatonin 20 mg/ day (forbidden in France at this dosage by the way) and that it could be of good help : sustains the immune system, anti-oxidant, anti inflammatory, leads to cancer cells suicide ... Should I add Melatonin to the treatment ? It seems also that Malatonin is non compliant with corticoids as it inhibits corticoids effects : what do you think ?

2. More globally I fear corticoids may have a negative impact on the different supplements. Is there a mean to sustitute it by anything else ? Is Celebrex an option ? If so at which dosage ? (the inflammation is still important, but decreasing).

3. Would you have any recommandation in other supplements or drugs ? PSK mushroom, Valpoic Acid, Metformin? 

Thank you very much for your precious help.

Saturday, 5 January 2019

Question about TP53 Mutated Tumors and Use of Chloroquine

In reviewing the section on TP53 from Astrocytoma Options, I noted "autophagy inhibition with chloroquine prevented restoration to normal P53 function" and therefore best to avoid autophagy inhibitors. Would someone kindly clarify whether this suggests chloroquine should be avoided for my husband who was found to have TP53 Variants including insertions/deletions? 
We have been using it for its purported benefit for EGFR amplification.
If so, are there other autophagy inhibitors to avoid?
Would Zinc be worth adding? (Tried it briefly in the past and experienced GI upset).

Many thanks and healing wishes to all--

Wednesday, 2 January 2019

Limited activity

My personal computer is out of service and I won’t be able to get a new one until next week at the earliest.  Consequently my blogging and researching abilities will be very limited until then. Thanks for your patience and best wishes to all in the new year!
Stephen