This was an orthotopic (intracranial) tumor implant model, with U87 (p53 wild-type) GBM cells, and TMZ and zinc were administered orally. The study does not say the dose of zinc used, and does not address whether zinc is normalizing the protein conformation of p53 in the U87 cells. A prior publication showed that U87 cells had mostly mutant-type dysfunctional conformation of the p53 protein, even though there was no p53 gene mutation, due to high expression of metallothionein which steals zinc from the p53 protein.
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